| 质粒名称: | pCMV-MCP-HA-WEEV nsP3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002871 |
| 产品用途: | Mammalian expression of MCP-HA-WEEV nsP3 |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | WEEV nsP3 |
| 物种来源: | Alphavirus |
| 密码子优化: | 大肠杆菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication
Molecular Cell, Volume 86, Issue 9, 2026
Yi Liu, Peiguo Yang
|
pCMV-MCP-HA-WEEV nsP3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-MCP-HA-WEEV nsP3载体质粒基本信息
pCMV-MCP-HA-WEEV nsP3质粒图谱载体图谱和pCMV-MCP-HA-WEEV nsP3载体序列质粒序列多克隆位点信息
pCMV-MCP-HA-WEEV nsP3质粒载体简介
pCMV-MCP-HA-WEEV nsP3质粒序列载体序列
LOCUS pCMV-MCP-HA-WEEV nsP3 5961 bp DNA circular SYN 16-JUL-2026
DEFINITION Localization: C1 Cloning Vector, Excitation: 488, Emission: 507.
ACCESSION .
VERSION .
KEYWORDS pCMV-MCP-HA-WEEV nsP3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5961)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 5961)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5961
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 142..445
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 446..649
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 600..620
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(672..708)
/label=引物 6
primer_bind 679..717
/label=引物 1
regulatory 688..693
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 694..1080
/codon_start=1
/label=MCP
/translation="MLAVKMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRS
QAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGL
LKDGNPIPSAIAANSGIYADPPVAT"
CDS 1093..1119
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(1117..1140)
/label=p61-1060
primer_bind 1126..1163
/label=p61-1055
CDS 1141..2718
/codon_start=1
/label=WEEV nsP3
/translation="APAYRVIRGDISKSADQAIVNAANSKGQPGSGVCGALYRKWPAAF
DRQPIAVGTARLVKHEPLIIHAVGPNFSKMPEPEGDLKLAAAYMSIASIVNAERITKIS
VPLLSTGIYSGGKDRVMQSLHHLFTAFDTTDADVTIYCLDKQWETRIIEAIHRKESVEI
LDDDKPVDIDLVRVHPNSSLAGRPGYSVNEGKLYSYLEGTRFHQTAKDIAEIHAMWPNK
SEANEQICLYILGESMSSIRSKCPVEESEASAPPHTLPCLCNYAMTAERVYRLRSAKKE
QFAVCSSFLLPKYRITGVQKLQCSKPVLFSGVVPPAVHPRKYAEIILETPPPPATTTVI
CEPTVPERIPSPVISRAPSAESLLSLGGVSFSSSATRSSTAWSDYDRRFVVTADVHQAN
TSTWSIPSAPGLDVQLPSDVTDSHWSIPSASGFEVRTPSVQDLTAECAKPRGLAEIMQD
FNTAPFQFLSDYRPVPAPRRRPIPSPRSTASAPPVPKPRRTKYQQPPGVARAISEAELD
EYIRQHSN"
misc_feature 2506..2541
/label=G3BP binding motif (hypothesized)
misc_feature 2650..2700
/label=FXR binding motif
primer_bind complement(2696..2733)
/label=p61-1056
primer_bind 2719..2744
/label=p61-1059
polyA_signal 2830..2951
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2867..2886)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2921..2940
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2958..3413)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3095..3116)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3307..3326
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3440..3544
/gene="bla"
/label=AmpR promoter
promoter 3546..3903
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3569..3589)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3754..3889
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3816..3835
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3839..3850)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3938..4732
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3992..4011)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4602..4621
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4920..4939)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4964..5011
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5340..5928
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5829..5848
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
61 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
661 ccgtcagatc cgctagcgct accggtcgcc accatgctag ccgttaaaat ggcttctaac
721 tttactcagt tcgttctcgt cgacaatggc ggaactggcg acgtgactgt cgccccaagc
781 aacttcgcta acgggatcgc tgaatggatc agctctaact cgcgttcaca ggcttacaaa
841 gtaacctgta gcgttcgtca gagctctgcg cagaatcgca aatacaccat caaagtcgag
901 gtgcctaaag gcgcctggcg ttcgtactta aatatggaac taaccattcc aattttcgcc
961 acgaattccg actgcgagct tattgttaag gcaatgcaag gtctcctaaa agatggaaac
1021 ccgattccct cagcaatcgc agcaaactcc ggcatctacg cggatccacc ggtcgccacc
1081 ccggtcgcca cctatccata tgacgttcca gattacgctt actcagatct cgagctcaag
1141 gcacccgcct atagagttat ccgcggcgat atctctaaga gtgcagacca ggccatcgtc
1201 aacgccgcta attccaaggg acagcctggc tctggtgtgt gtggcgctct ctataggaag
1261 tggcccgctg ctttcgaccg gcagcctatc gcagtgggaa cagcccgact ggtcaaacat
1321 gaaccactga tcatccacgc cgtggggccc aacttctcta aaatgcctga gcctgaaggc
1381 gatctcaagc tcgctgctgc atacatgagt attgccagca ttgttaatgc tgaaagaatc
1441 acaaaaatat ccgtccctct gctgagtacc ggaatttact ctggaggtaa ggacagggtg
1501 atgcagagcc tgcaccacct gtttaccgcg tttgacacga ccgacgccga cgttacgatc
1561 tattgtttgg ataaacagtg ggaaacaagg attatcgaag ctatacacag gaaggagtca
1621 gtggagattc tggatgatga caagcctgtg gacatagacc tggttcgagt tcatcccaat
1681 tcaagcctgg ctggccgccc tggatactct gtcaatgagg ggaagttgta tagctatttg
1741 gagggtacgc gcttccatca gacagctaaa gatatcgctg aaatccatgc tatgtggcct
1801 aacaagtctg aagcaaatga gcagatctgt ctctatattc tgggcgaaag tatgagtagc
1861 atcagatcca aatgtcctgt cgaagaatct gaagcctctg caccccccca tactctccca
1921 tgcttgtgta attacgcaat gaccgccgaa cgcgtataca gattgaggag tgctaagaaa
1981 gagcaatttg ctgtgtgttc ttcttttctg cttcccaaat acaggattac cggcgtgcag
2041 aagttgcagt gttcaaaacc tgtgctcttt tcaggagtcg tgcctccagc cgtgcatcca
2101 aggaaatacg ccgaaatcat ccttgagaca ccccccccac cggctaccac tacagtgata
2161 tgcgagccaa cagttccgga gcgcattcca agccccgtga ttagccgcgc tccatcagct
2221 gaatcactgc tttccctggg cggggtgtct ttttcaagca gcgccacaag gagtagcacg
2281 gcgtggtctg attacgatcg gaggtttgtc gtgacagcgg atgttcatca ggctaacacc
2341 tctacttgga gtatccctag cgcccctgga cttgatgtac agctgccaag cgacgtgacg
2401 gacagccact ggagtatacc gagtgcttca ggatttgaag tccgaacacc gtctgtccaa
2461 gatctgacag ccgaatgtgc caaacccaga ggtctcgctg aaatcatgca agatttcaac
2521 acggcccctt tccagttcct cagtgattat agacccgtgc cagcacctag acggaggcca
2581 atcccctcac caagatcaac tgcttccgca cctcctgtcc cgaaaccaag acgcaccaag
2641 tatcagcagc cacccggggt cgctagggct attagtgagg ccgagctcga cgagtacata
2701 agacaacact caaactgata actgatcata atcagccata ccacatttgt agaggtttta
2761 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
2821 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
2881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
2941 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
3001 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
3061 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
3121 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
3181 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
3241 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
3301 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
3361 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
3421 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
3481 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
3541 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
3601 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
3661 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
3721 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
3781 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
3841 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
3901 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
3961 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
4021 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
4081 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
4141 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
4201 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
4261 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
4321 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
4381 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
4441 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
4501 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
4561 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
4621 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
4681 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
4741 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
4801 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
4861 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
4921 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
4981 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
5041 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
5101 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
5161 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
5221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
5281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
5461 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
5521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
5581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
5641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
5701 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
5761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
5821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
5881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
5941 gcggcctttt tacggttcct g
//
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